Plant Ontology and Gramene conducted a Gene Annotation Workshop at the ASPB 2010 conference in Montreal. It was well attended and participants had the opportunity to do hands on annotation exercises using plant and gene ontologies. Presentations from representatives from TAIR, BAR and MaizeGDB introduced the respective databases and highlighted their annotation efforts. Approximately 40 scientists attended the workshop, and 14 of the participants received travel awards from Gramene.