We are pleased to announce the 31st release of the Gramene database which includes updates to our genomes, diversity, maps, pathways, and web services. Our genome browser has been updated to Ensembl version 57 and hosts fourteen genomes with new annotations, Fgenesh gene predictions, gene trees, whole genome alignments, and synteny views. The genetic diversity databases for rice, Arabidopsis, and maize have new data sets; there are helpful links to start external tools such as TASSEL and Flapjack from our website; and our SNP query tool now shows gene loci overlapping SNP positions. We have three new genetic maps, an updated RicyCyc based on the MSU6 assembly, a new PoplarCyc mirror, a redesigned home page, and greatly expanded DAS options. For more details, please see our build 31 release notes. If you have comments, feel free to use use our feedback form or write us at gramene@gramene.org.